Introduction:

This tool performs the Student t-test and multiple comparison correction on the peaks of the inputted table. Group information is given by a group design file (Tab-delimited text file). The number of groups should be 2. For paired t-test, pairs are defined according to the order in each of the two groups and the number of samples must be equal in the two groups.

Input files:

1. Peak table file in Tab-delimited txt format, first column is the compound identifier, the others are samples.

For example:

	HU_011	HU_014	HU_015	HU_017	HU_018	HU_019
(2-methoxyethoxy)propanoic acid isomer	3.019766	3.814339	3.519691	2.562183	3.781922	4.161074
(gamma)Glu-Leu/Ile	3.888479	4.277149	4.195649	4.32376	4.629329	4.412266
1-Methyluric acid	3.869006	3.837704	4.102254	4.53852	4.178829	4.516805
1-Methylxanthine	3.717259	3.776851	4.291665	4.432216	4.11736	4.562052
1,3-Dimethyluric acid	3.535461	3.932581	3.955376	4.228491	4.005545	4.320582
1,7-Dimethyluric acid	3.325199	4.025125	3.972904	4.109927	4.024092	4.326856
2-acetamido-4-methylphenyl acetate	4.204754	5.181858	3.88568	4.237915	1.852994	4.080681
2-Aminoadipic acid	4.080204	4.359246	4.249111	4.231404	4.323679	4.244485

2. Group design file. For paired t-test, pairs are according to the order in each of the two groups.

For example: the group file for paired t-test, with 3 pairs (_p1, _p2, and _p3) in different color blocks

Output files:

1. 't_test_results.txt', T-test results with pvalue, log2FC, and qvalue.

2. 't_test_significant_results.txt', significant T-test results.

Note:

Groups number must be 2 in the sample group file.

Group names of characters or string are preferred. Numbers are also supported but not recommended.