Introduction:

This tool takes a peak table file as input and performs internal standard (IS) normalization within samples. The normalization formula is (x/internal standard) *10000.

Input files:

1. Peak table file in Tab-delimited text format with the first column as the compound identifier and others as samples.

For example:

AlignID	STDmix_GC_01	STDmix_GC_02	STDmix_GC_03
1	1486892478	561322777	3448620272
Nitrogen dioxide	5492977592	684434115	3265669981
Ethanol, 2-fluoro-	2265686433	4182838129	4365291513
3-Pentanone, 2,2,4,4-tetramethyl-	13390154	12612932	21155307
Hydrazine	14588107	8510918	7224351

Parameters:

Set the standard compound. Chlorophenylalanine

Output files:

1. 'internal_stdNorm_peak_table.txt', internal standard normalized peak table file in Tab-delimited txt format.

Note:

1. The IS compound must exist in the inputted peak table.

2. Experimental preparation: The internal standard must be prepared in advance and added quantitatively to each sample.